Posts
Obtaining Insect-resistant Cotton by Transformation with Agrobacterium
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT A plant expression vector, pBinLK, carrying two insecticidal genes: pea lectin gene and soybean Kunitz trypsin inhibitor gene, were constructed and successfully transferred into 4 upland cotton (Gossypium hirsutum L.) cultivars, Xinluzao-1, Xinluzhong-2, Jihe-321 and Liao-9 via Agrobacterium-mediated transformation. Hypocotyl segments fro...
Response to In Vitro Regeneration of Immature Zygotic Embryos in Cotton (Gossypium spp)
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT The in vitro culture response of immature zygotic embryos (IZE), from six Gossypium hirsutum and one G. barbadense variety, was studied. Explants were collected from plants grown in the field and the greenhouse. Bolls were collected 12, 14 and 16 days after pollination (DAP) from the field and 10, 12 and 14 DAP from the greenhouse. The yo...
Random Amplified Polymorphic DNA Studies in Cotton
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT Polymerase chain reaction (PCR)-based random amplified polymorphic DNA (RAPD) analysis can to be an important tool in the hands of plant breeders. Among its many applications, RAPD analysis can be used to measure genetic diversity and for DNA fingerprinting. In this investigation, 22 genotypes, representing cultivated Gossypium hirsutum,...
Genetics and Combining Ability of Leaf Curl Virus Resistance in Cotton (Gossypium hirsutum L.)
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT The cotton leaf curl virus (CLCV) disease caused by Gemini viruses has resulted in failure of the cotton crop in many countries. The losses caused by this disease range from 20 to 80 percent depending upon the stage of viral infection by virulent cotton white fly (Bemisia tabaci). The only permanent approach of combating this menace is th...
Development of Transgenic Insect-protected Cotton Plants
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT An artificially synthesized Bacillus thuringiensis insecticidal protein gene, co-constructed with the GUS reporter gene (Bt/GUS), was transferred into fertilized ovaries of the elite cotton (Gossypium hirsutum L.) cultivars Simian 3 and Zhongmiansuo 12 by the pollen tube pathway. Transgenic cotton plants were recovered from the seeds in t...
Induction of Multiple Shoot Differentiation and Increasing Transformation Frequency in Shoot Tips of Egyptian Cotton
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT Multiple shoot differentiation and regeneration from cotton (Gossypium barbadense L.) shoot tips of aseptically-grown seedlings was developed on Murashige and Skoog media supplemented with 100 mg/L myo-inositol, 1.0 mg/L thiamin-HCL, 2% glucose, 1X Gamborg vitamin, 100 mg/L citric acid, 100 mg/L ascorbic acid, 0.1 mg/L kinetin, various le...
Transformation of Egyptian Cotton Tissue (Gossypium barbadense) Using Agrobacterium tumefaciens
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT Callus induction from hypocotyl and cotyledonary explants of Egyptian cotton (Gossypium barbadense), extra-long staple (Giza 45, Giza 77) and long staple (Giza 85), were evaluated for in vitro callus induction and maintenance utilising two media formulations (G2) and (CI). Giza 85 produced significantly better callus than Giza 45 and Giza...
Utilization of Cotton Haploids in Basic and Applied Research
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT Systematic cytological work in the genus Gossypium L. resulted in isolation of haploids (2n=2x=26) in tetraploid (2n=4x=26) G. hirsutum, G. barbadense and their F2 interspecific crosses and monoploid (n=x=13) cultivated Asiatic (2n=2x=26) cotton. The haploids and monoploids were studied for differences in their morphological characters at...
Development of an Efficient Regeneration and Transformation System for Commercially Grown South African Cotton Cultivars
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT While establishing a system for genetic transformation of South African cotton cultivars, a method of regeneration and transformation of the shoot apical meristem of seedlings was developed. Shoot apices from young cotton seedlings (Gossypium hirsutum cvs Sabie, Jassid, Palala and 107/1) were placed onto Murashige and Skoog (MS) basal med...
Haploid Induction in Cotton: A Future Perspective
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT The value of haploids in genetic analysis and plant breeding has long been known. This is more so in cotton, since the production of double haploids in large numbers could open the way for the development of partial interspecific hybrids between Gossypium hirsutum and G. barbadense. For the induction of haploids in cotton three approaches...
Confocal Imaging on Microprojectile-bombarded Pollen of Gossypium hirsutum L.
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT Confocal laser scanning microscopy (CLSM) was introduced to study transgenic molecular cytobiology. Scanning electron microscopy (SEM) was used to observe the transferring traces of accelerated, DNA-coated, metal particles labeled with 4,6-diamidino-2-phenylindole (DAPI) in mature cotton pollen (G. hirsutum L.). The results indicated t...
Pollen-mediated Transformation by Microprojectile Bombardment is Genotype Class Independent in Gossypium hirsutum L.
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT The biological characteristics of bombarded pollen were presented by the optimized delivery mode for TM-1, a genetic standard, and 23 Chinese G. hirsutum cultivars, which were collected from 5 ecological regions and derived from 7 ancestors. The results showed that, compared with unbombarded pollen, the average percentage of GUS transient...
Optimization of Microprojectile Bombardment Parameters for Pollen-Mediated Transformation in Gossypium hirsutum L.
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT Mature pollen-mediated transformation in cotton (Gossypium hirsutum L.) by microprojectile bombardment was established under gaseous medium in our laboratories. Bombardment parameters influencing the vitality, in vitro germinability and GUS transient expression of bombarded pollen were studied and optimized by the Gray System Analysis met...
Efficient Protoplast Isolation Towards an Adapted Culture Protocol for Cotton (Gossypium sp.)
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT The media composition for successful cotton (Gossypium sp.) protoplast isolation and cultivation was determined through systematic investigation of all the components of each culture medium. A set of orthogonal design experiments was used to verify the effect of each compound of the initial medium alone and in combination with other compo...
Cotton Biotechnology in Uzbekistan
By
Muhammad Naveed Arshad
on
Oct 8, 2018
ABSTRACT The Republic of Uzbekistan is a large producer and exporter of cotton but must overcome a number of limitations. Soil salinity has rendered about 20 % of the cotton producing area unsuitable for continued production. Increasing susceptibility to pathogens and pests reduces the yield by 10 – 12 %. Defoliants and harvest aid chemicals incre...
Effects of Foliar Applied Potassium on Cotton in the San Joaquin Valley of California
By
Muhammad Naveed Arshad
on
Sep 20, 2018
ABSTRACT Foliar fertilization of cotton, Gossypium hirsutum and G. barbadense, has been a subject of researchers since 1992 in California. The practice has expanded from its limited beginnings to widespread use for supplying supplemental nitrogen and potassium. Yield and economic advantages have been realized by researchers, and growers alike. Rep...
Nitrogen Fertilization of Cotton Based on Inorganic Nitrogen Analysis of the Soil
By
Muhammad Naveed Arshad
on
Sep 20, 2018
ABSTRACT Cotton is produced on widely differing soils under irrigated or rainfed conditions in South Africa. Under these circumstances seed cotton yields can vary greatly and so do their nitrogen demands. This model was the culmination of nitrogen field trials carried out over three seasons at four locations scattered over South Africa. In these t...
Influence of Nitrogen Nutrition on the Yield and Quality of Cotton
By
Muhammad Naveed Arshad
on
Sep 20, 2018
ABSTRACT Nitrogen plays an important role in the growth and yield of cotton. In rigid genetical conditions, growth and development of different cotton cultivars react differently to nitrogen fertilizers. The changes in parameters of cotton fibers of different cotton cultivars that react to the application of increasing doses of nitrogen fertilizer...
The Effects of the Nitrogen Fertilizer Rates on the Uptake and Yield of Cotton on Calcareous Soil
By
Muhammad Naveed Arshad
on
Sep 12, 2018
ABSTRACT Excessive nitrogen fertilizer rates in Upland cotton (Gossypium hirsutum L.) produces high levels of nitrates in calcareous soils. The experiments were conducted in the Experimental Station of Vardates in central Greece, during the seasons 1992 and 1993. The objectives were to examine the effects of variable nitrogen rates on the nitrogen...
The Effect of Potassium Fertilizer Plus Higher Nitrogen Rates on Growth and Yield of Cotton
By
Muhammad Naveed Arshad
on
Sep 12, 2018
ABSTRACT Two field experiments were carried out in two locations of Beni-Suef Governorate in middle Egypt, on Giza 75 cotton cultivar (Gossypium barbadense L.) to increase cotton yield by increasing nitrogen rates, while enhanced by potassium. The treatments were 112.5, 150, 187.5 kg N/ha and 187.5, 225.5 kg N plus 60kg K/ha. Results revealed that...