Genome-wide analysis of Rf-PPR-like (RFL) genes and a new InDel marker development for Rf1 gene in cytoplasmic male sterile CMS-D2 Upland cotton
By Quanyi LIU on Dec 14, 2018

Breeding & Genetic improvement Biotechnology Breeding JCR

[Background] Cytoplasmic male sterility in flowering plants is a convenient way to use heterosis via hybrid breeding and may be restored by nuclear restorer-of-fertility (Rf) genes. In most cases, Rf genes encoded pentatricopeptide repeat (PPR) proteins and several Rf genes are present in clusters of similar Rf-PPR-like (RFL) genes. However, the Rf...

A genome-wide analysis of SWEET gene family in cotton and their expressions under different stresses
By Quanyi LIU on Dec 13, 2018

Breeding & Genetic improvement Biotech-global JCR

[Background] The SWEET (Sugars will eventually be exported transporters) gene family plays multiple roles in plant physiological activities and development process. It participates in reproductive development and in the process of sugar transport and absorption, plant senescence and stress responses and plant-pathogen interaction. However, the comp...

Hypoxia tolerance studies for yield, fiber and physiological traits in cotton (Gossypium hirsutum L.)
By Quanyi LIU on Dec 11, 2018

Agro-physio-Asia Breeding-Asia Breeding JCR

[Background] Hypoxia tolerance studies in cotton are very rare in Pakistan. Unpredicted and excessive rainfalls result in severe losses to cotton crop in many regions of the country due to lack of hypoxia tolerance in current cotton varieties. The genotypes that can tolerate flooding are not reported earlier. The studies were conducted to explore h...

Efficiency of cotton bollworm (Helicoverpa armigera Hübner) control of different Bt cotton varieties in North China
By Quanyi LIU on Dec 11, 2018

Croprotection-Asia JCR

[Background] The cotton bollworm (Helicoverpa armigera) is one of cotton’s most destructive insect pests in terms of yield and quality. Since 1997, China has grown commercially available transgenic Bacillus thuringiensis (Bt) cotton. We aimed to investigate the variation in resistance of transgenic Bt cotton varieties to cotton bollworm in North Ch...

Comparative transcriptome study provides insights into acquisition of embryogenic ability in upland cotton during somatic embryogenesis
By Quanyi LIU on Dec 11, 2018

Biotechnology JCR

[Background] The conversion from non-embryogenic callus (NEC) to embryogenic callus (EC) is the key bottleneck step in regeneration of upland cotton (Gossypium hirsutum), and hinders the transgenic breeding of upland cotton. To investigate molecular mechanisms underlying acquisition of embryogenic potential during this process, comparation analysis...

Genotypic variation in spatiotemporal distribution of canopy light interception in relation to yield formation in cotton
By Quanyi LIU on Dec 10, 2018

Agro-physio-Asia JCR

[Background] Within-canopy interception of photosynthetically active radiation (PAR) impacts yield and other agronomic traits in cotton (Gossypium hirsutum L.). Field experiments were conducted to investigate the influence of 6 cotton varieties (they belong to 3 different plant types) on yield, yield distribution, light interception (LI), LI distri...

Saponin synthesis and cotton growth is antagonistically regulated by solar UV-B radiation
By Quanyi LIU on Dec 9, 2018

Agro-physio-Asia JCR

An article of Journal of Cotton Research has been published online. [Background] Earlier we have reported that the exclusion of solar UV-B increased the growth and yield of cotton plants as compared with ambient UV-B. The UV-B radiation effects on the saponins and the impact of ambient and reduced UV-B on the accumulation of saponins has not bee...

Comparative Efficiency of Three Selection Methods for Yield and Quality of Cotton (Gossypium hirsutum L.)
By Muhammad Naveed Arshad on Nov 2, 2018

WCRC WCRC2 Breeding

ABSTRACT The comparative efficiency of three selection methods, honeycomb pedigree selection (HC), pedigree selection (PS) and single-seed descent (SSD), was assessed in an F2 cotton population in two locations. The best five lines derived by each method in each location after five cycles of selection, along with check varieties were tested in com...

Obtaining Insect-resistant Cotton by Transformation with Agrobacterium
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT A plant expression vector, pBinLK, carrying two insecticidal genes: pea lectin gene and soybean Kunitz trypsin inhibitor gene, were constructed and successfully transferred into 4 upland cotton (Gossypium hirsutum L.) cultivars, Xinluzao-1, Xinluzhong-2, Jihe-321 and Liao-9 via Agrobacterium-mediated transformation. Hypocotyl segments fro...

Response to In Vitro Regeneration of Immature Zygotic Embryos in Cotton (Gossypium spp)
By Muhammad Naveed Arshad on Oct 8, 2018

WCRC WCRC2 Biotechnology

ABSTRACT The in vitro culture response of immature zygotic embryos (IZE), from six Gossypium hirsutum and one G. barbadense variety, was studied. Explants were collected from plants grown in the field and the greenhouse. Bolls were collected 12, 14 and 16 days after pollination (DAP) from the field and 10, 12 and 14 DAP from the greenhouse. The yo...

Random Amplified Polymorphic DNA Studies in Cotton
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT Polymerase chain reaction (PCR)-based random amplified polymorphic DNA (RAPD) analysis can to be an important tool in the hands of plant breeders. Among its many applications, RAPD analysis can be used to measure genetic diversity and for DNA fingerprinting. In this investigation, 22 genotypes, representing cultivated Gossypium hirsutum,...

Genetics and Combining Ability of Leaf Curl Virus Resistance in Cotton (Gossypium hirsutum L.)
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT The cotton leaf curl virus (CLCV) disease caused by Gemini viruses has resulted in failure of the cotton crop in many countries. The losses caused by this disease range from 20 to 80 percent depending upon the stage of viral infection by virulent cotton white fly (Bemisia tabaci). The only permanent approach of combating this menace is th...

Development of Transgenic Insect-protected Cotton Plants
By Muhammad Naveed Arshad on Oct 8, 2018

WCRC WCRC2 Biotechnology

ABSTRACT An artificially synthesized Bacillus thuringiensis insecticidal protein gene, co-constructed with the GUS reporter gene (Bt/GUS), was transferred into fertilized ovaries of the elite cotton (Gossypium hirsutum L.) cultivars Simian 3 and Zhongmiansuo 12 by the pollen tube pathway. Transgenic cotton plants were recovered from the seeds in t...

Induction of Multiple Shoot Differentiation and Increasing Transformation Frequency in Shoot Tips of Egyptian Cotton
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT Multiple shoot differentiation and regeneration from cotton (Gossypium barbadense L.) shoot tips of aseptically-grown seedlings was developed on Murashige and Skoog media supplemented with 100 mg/L myo-inositol, 1.0 mg/L thiamin-HCL, 2% glucose, 1X Gamborg vitamin, 100 mg/L citric acid, 100 mg/L ascorbic acid, 0.1 mg/L kinetin, various le...

Transformation of Egyptian Cotton Tissue (Gossypium barbadense) Using Agrobacterium tumefaciens
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT Callus induction from hypocotyl and cotyledonary explants of Egyptian cotton (Gossypium barbadense), extra-long staple (Giza 45, Giza 77) and long staple (Giza 85), were evaluated for in vitro callus induction and maintenance utilising two media formulations (G2) and (CI). Giza 85 produced significantly better callus than Giza 45 and Giza...

Utilization of Cotton Haploids in Basic and Applied Research
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT Systematic cytological work in the genus Gossypium L. resulted in isolation of haploids (2n=2x=26) in tetraploid (2n=4x=26) G. hirsutum, G. barbadense and their F2 interspecific crosses and monoploid (n=x=13) cultivated Asiatic (2n=2x=26) cotton. The haploids and monoploids were studied for differences in their morphological characters at...

Development of an Efficient Regeneration and Transformation System for Commercially Grown South African Cotton Cultivars
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT While establishing a system for genetic transformation of South African cotton cultivars, a method of regeneration and transformation of the shoot apical meristem of seedlings was developed. Shoot apices from young cotton seedlings (Gossypium hirsutum cvs Sabie, Jassid, Palala and 107/1) were placed onto Murashige and Skoog (MS) basal med...

Haploid Induction in Cotton: A Future Perspective
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT The value of haploids in genetic analysis and plant breeding has long been known. This is more so in cotton, since the production of double haploids in large numbers could open the way for the development of partial interspecific hybrids between Gossypium hirsutum and G. barbadense. For the induction of haploids in cotton three approaches...

Confocal Imaging on Microprojectile-bombarded Pollen of Gossypium hirsutum L.
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT Confocal laser scanning microscopy (CLSM) was introduced to study transgenic molecular cytobiology. Scanning electron microscopy (SEM) was used to observe the transferring traces of accelerated, DNA-coated, metal particles labeled with 4,6-diamidino-2-phenylindole (DAPI) in mature cotton pollen (G. hirsutum L.). The results indicated t...

Pollen-mediated Transformation by Microprojectile Bombardment is Genotype Class Independent in Gossypium hirsutum L.
By Muhammad Naveed Arshad on Oct 8, 2018

Biotechnology WCRC WCRC2

ABSTRACT The biological characteristics of bombarded pollen were presented by the optimized delivery mode for TM-1, a genetic standard, and 23 Chinese G. hirsutum cultivars, which were collected from 5 ecological regions and derived from 7 ancestors. The results showed that, compared with unbombarded pollen, the average percentage of GUS transient...