Comparative chloroplast genome sequence analyses of gossypium thurberi with two cultivated allotetraploid gossypium species
Jun 16, 2019
Biomol - Biotechnology Network coordination Network-MedMiddleEast Biomol - Biotech-Asia
West Azerbaijan Agricultural and Natural Resources Research Center, AREEO, Urmia, Iran
The chloroplast is the sole site for photosynthetic activities in green plants and contains the whole enzymatic machinery needed for this purpose. Gossypium thurberi is a wild diploid species and also one of the most important taxa in the genus which widely has been used to improve cultivated allotetraploid cotton with its great potential of genetic resources. Present study was conducted in order to analyse sequence of its chloroplast. The thurberi chloroplast (cp) genome is 160264 bp in length which has conserved quadripartite structure. Single copy region of cp genome is separated by the two inverted regions. The large single copy region is 88,737 bp, and the small single copy region is 20,271 bp whereas the inverted repeat 25,628 bp each. The genome has 113 single genes and 20 duplicated genes. These single genes comprised of 79 protein coded genes, 4 ribosomal RNA genes and 30 transfer RNA genes. Out of all the genes, 18 genes have 1 or 2 introns. As Compared to cpDNA from the two cultivated allotetraploid Gossypium species, rps18 was duplicated only in thurberi.
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