Confocal Imaging on Microprojectile-bombarded Pollen of Gossypium hirsutum L.
Oct 8, 2018

Biomol - Biotechnology WCRC WCRC2
Confocal laser scanning microscopy (CLSM) was introduced to study transgenic molecular
cytobiology. Scanning electron microscopy (SEM) was used to observe the transferring traces
of accelerated, DNA-coated, metal particles labeled with 4,6-diamidino-2-phenylindole
(DAPI) in mature cotton pollen (G. hirsutum L.). The results indicated that pollen exine can
be penetrated by the high-velocity microprojectiles, and the microprojectile with fluorescent
probe and its transferring trace in the exine-detached pollen can be observed. The optical
sectioning imaging analysis indicated that the microprojectile can be introduced into a
bombarded pollen grain approximately 20.6 m deep. In bombarded grains, transient
expression of a β-glucuronidase (GUS) gene in pBI121 was certified by histochemical assay.

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