DEVELOPMENT OF LABORATORY AND FIELD ELISA TESTS FOR DETECTION OF ENDOSULFAN IN WATER AND SOIL
Mar 13, 2017

Crop Protection WCRC Croprotection-australia WCRC1
Abstract                                                                         Back to Table of contents

Environmental monitoring of endosulfan residues is currently being achieved by gas chromatographic analysis, but there is a need for faster and cheaper analysis that can be conducted in the field as well as in the laboratory. To satisfy this need, two enzyme immunoassay methods, a laboratory assay based on microwell plates and a field test based on the use of small plastic tubes, have been developed for the detection of endosulfan residues in water and soil. These assays have the detection limit of 0.2 ppb of endosulfan, and the detection range of 0.2 ppb - 10 ppb for laboratory assay and 0.2 ppb - 20 ppb for field assay. The field test takes 15 minutes to complete using dropper bottles containing enzyme conjugate and colour development reagents, and can be performed at the site of possible contamination. The laboratory test takes longer to run, but the ability to analyse large numbers of samples simultaneously gives the method high throughput. Water samples can be analysed directly and soil samples are simply extracted with 90% methanol. The tests also detect endosulfan sulfate with similar sensitivity to endosulfan but are four to ten times less sensitive to endosulfan diol, and therefore can potentially determine endosulfan and endosulfan sulfate from the total endosulfan residues present in the environment.

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