Haploid Induction in Cotton: A Future Perspective
Oct 8, 2018
The value of haploids in genetic analysis and plant breeding has long been known. This is more so in cotton, since the production of double haploids in large numbers could open the way for the development of partial interspecific hybrids between Gossypium hirsutum and G. barbadense. For the induction of haploids in cotton three approaches were applied, namely: anther culture, induction of parthenogenesis after pollination of cotton flowers with alien pollen, and semigamy. In vitro culture of anthers originated from 10 cotton varieties and a number of intraspecific and interspecific cotton hybrids resulted only in the production of callus with a number of haploid cells and very few embryoids. Haploid and double haploid plants were obtained in a relatively small number after pollination of a semigametic line with pollen from interspecific cotton hybrids. Pollination of cotton flowers with pollen from related species (Hibiscus syriacus, Abelmoschus esculentus, Malva sylvestris, Datura stramonium, Hibiscus cannabinus) and in vitro culture of young ovules resulted in the production of abnormal plantlets when A. esculentus and D. stramonium were the pollinators and of mature cotton plants when the pollinator was H. cannabinus.