INDUCTION OF SOMATIC EMBRYOGENESIS IN A RANGE OF COTTON CULTIVARS
Mar 10, 2017

Breeding & Genetic improvement WCRC Breeding-NorthAmerica WCRC1
Abstract                                                                         Back to Table of contents

Diverse genotypes of cotton Gossypium hirsutum L. were evaluated for their capacity to form callus formation and for induction of somatic embryogenesis.  Callus cultures were evaluated from hypocotyl, mesocotyl and cotyledonary leaf explants, harvested from in-vitro germinated seedlings.  For callus induction eight different initiation media were tested.  Maintenance medium for all the cultures was the same, used initially for callusing.

After a series of sub-cultures, calli were transferred into embryo initiation medium which consist of Murashige and Skoog (1962) basal medium, Gamborg B5 vitamins, glucose (3%) and agar (0.6%).  After several weeks, calli were screened for the presence of embryonic units.  The embryogenic tissue were isolated and sub-cultured on embryo-proliferation medium (Finer, 1988).  After 4-6 weekly sub-culture, three different cell lines were obtained from the hypocotyl derived callus in cv PKV 081, which was previously identified as being highly embryogenic.  Microscopic preparation showed the presence of somatic embryos in various stages of development.  In one cell line derived from Coker 417-68, differentiation of a shoot was observed.

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