SCREENING OF SOMATIC MUTANTS FOR RESISTANCE TO VERTICILLIUM WILT IN GOSSYPIUM HIRSUTUM L.
Mar 13, 2017

Breeding & Genetic improvement WCRC Breeding-Asia WCRC1
Abstract                                                                         Back to Table of contents

Growth and phytotoxin-producing conditions of the Tianman isolate of V. dahliae were studied showing that filtrates of the pathogen cultures were able to make cotton seedlings wilt or die.  Furthermore, the filtrates decreased seed germination rate, increased penetrability of leaf discs and reduced the survival rate of callus.  Large differences existed between resistant and susceptible varieties in the above aspects.

Callus formed from mutated explants was used to select tolerant mutants using toxic filtrates as the screening factor.  Some tolerant cell lines and tolerant plants were obtained.

Thirty two plantlets were obtained from all the tolerant cell lines, and their resistance was tested.  On the medium with toxic filtrates, the tolerant plantlets showed low symptoms of the disease.

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