Use of Male Sterility in Cotton and Molecular markers for Fertility Restoration in CGMS of G.hirstum Cotton
Jun 16, 2019
Breeding & Genetic improvement Biomol - Biotechnology Network coordination Network-MedMiddleEast Biomol - Biotech-Asia Breeding-Asia
I.S. Katageri, *Rashmi Ranjan Toppo, and B.M. Khadi
*Department of Biotechnology, College of Agriculture University of Agricultural Sciences, Dharwad - 580 005, India
Cotton is one among the few often cross-pollinated crops, where the achievement of commercial exploitation of heterosis is comparable to even that of cross-pollinated crops like maize. The main bottleneck in exploitation of hybrid vigour in cotton is the complicated technique of seed production, which involves hand emasculation and pollination that makes hybrid cotton seed more costly. To improve the remunerative value of hybrid seed production and commercial cultivation of hybrid it is necessary to develop productive male sterile based hybrids. Although Cytoplasmic Genetic Male Sterility is available, the identification of restorer for 100 percent fertility restoration was problematic. Molecular markers are coming handy in identifying such plants. The present study, therefore, comprised 413 individual plants comprising of 164 A-line and B-line, 124 IPS of R-line, 13 hybrids, 3 cultivars, 4 wild species, 24 G. hirsutum germplasm lines, 80 RILs and 1 GMS line were studied during 2012-13 and 2013-14 at ARS, Dharwad farm. Twenty four molecular markers known to be associated with fertility restoration (10 RAPD, 7 SSR, 6 STS, and 1 TRAP) were studied. Out of these, 3 STS markers (Y1107, UBC 147 and UBC 607) were able to amplify only in 44 plants out of 124 belong to DR-7 (known for fertility restoration). No amplification was observed in diverse genetic background of A-lines and their respective B-lines, wild species, G. barbadense var. SBYF 425, G. arboreum var. DLSa 17, G. herbaceum var. Jayadhar, G. hirsutum germplasm lines and Abadhita GMS line (sterile and its maintainer line). However amplification by these three markers was recorded in 6 out of 80 RILs of DCH-32 hybrids. Co-segregation for 98-100 percent fertility restoration and molecular markers associated was observed in F2 of cross between A line and fertility restorer carrying three molecular markers.
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